Evaluation of peripheral blood neutrophil survival following �treatment with Mesenchymal stem cells in rats

Hamounnavard, Sahar and Delirezh, Nowruz and AfzaAhangaran, Nahideh (2014) Evaluation of peripheral blood neutrophil survival following �treatment with Mesenchymal stem cells in rats. Journal of Mazandaran University of Medical Sciences, 24 (112). pp. 34-40.

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Official URL: http://jmums.mazums.ac.ir/article-1-3056-en.html

Abstract

Background and purpose: Because of theirmultipotency and ease of purification, amplification and �immunomodulatory properties Mesenchymal stem cells (MSC), are anideal stem cell source for cell therapies. �This study was done to investigat the effect of Rat MSCs on the survival neutrophils.� Materials and methods: MSCs was isolated from rat (6-8 weeks) femoral and tibial bone marrow and �cultured in DMEM. Then maturation MSCs were incubated with neutrophils isolated from peripheral blood of �rat at 37 ° C for 1 h. Neutrophil survival was measured at 6 and 24 h incubation with of MSCs by flow �cytometric analysis using An/PI. Data were analyzed by SPSSsoftware, followed by Tukeytest at a significance �level of (P<0.05).� Results: The 6-hour incubation of neutrophils with MSCs, significantly reduced the percentage of �normal cells and increased apoptosis compared to controls and increased cell necrosis was not significant in �the treated group than in control. The 24-hour incubation of neutrophils with MSCs, significantly increased the �percentage of healthy cells and apoptosis was reduced compared to the control group, and reduced cell �necrosis was not significant in the treated groups than in control (p<0.05).� Conclusion: In spite of the clinical importance of MSCs, some biological aspects of them including �their self-renewal, proliferation and immune modulatory effects are of great therapeutic potential for cell �therapy.�

Item Type: Article
Uncontrolled Keywords: Mesenchymal stem cells, Neutrophil, Survival
Depositing User: Unnamed user with email eprints@mazums.ac.ir
Date Deposited: 04 Jan 2018 19:02
Last Modified: 04 Jan 2018 19:02
URI: http://eprints.mazums.ac.ir/id/eprint/762

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