Adiponectin Effects on Osteonectin Gene Expression in Vascular Smooth Muscle Cell Line

Niknam, Sara and GhatrehSamani, Keihan and Amini, Seyed Asadollah and Farokhi, Effat (2014) Adiponectin Effects on Osteonectin Gene Expression in Vascular Smooth Muscle Cell Line. Journal of Mazandaran University of Medical Sciences, 23 (109). pp. 216-222.

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Official URL: http://jmums.mazums.ac.ir/article-1-3299-en.html

Abstract

Background and purpose: Atherosclerosis is a major cause of death in adults in most countries. Many studies have focused on the protective role and anti-inflammatory properties of adiponectin but its role in calcification has been less studied. Studies that could determine the causes and mechanisms of calcification could be of great value. The aim of this study was to investigate the effects of adiponectin on osteonectin gene expression, a protein involved in vascular calcification. Materials and methods: In this experimental study, vascular smooth muscle cells were obtained from Pasteur Institute of Iran and were cultured in F12K medium containing β-glycerophosphate as calcifying stimuli. The cells were treated with 5μg/ml adiponectin. At 24 and 48 hours osteonectin gene expression in these cells was studied against control cells using real time PCR. Results: We observed that adiponectin increased osteonectin gene expression against control cells 2.17 and 3.6 fold in vascular smooth muscle cells at 24 and 48 hours, respectively. Conclusion: Increasing the expression of osteonectin gene lead to increases in calcification, therefore, adiponectin could be considered as a risk factor for atherosclerosis.

Item Type: Article
Uncontrolled Keywords: Atherosclerosis, vascularsmooth muscle cells, adiponectin, osteonectin.
Depositing User: Unnamed user with email eprints@mazums.ac.ir
Date Deposited: 04 Jan 2018 19:09
Last Modified: 04 Jan 2018 19:09
URI: http://eprints.mazums.ac.ir/id/eprint/599

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