Preparation and preliminary studies of 64Cu-antiMUC-1 for breast cancer targeting

Alirezapour, Behrouz and Rasaee, Mohammad Javad and Jalilian, Amir Reza and Paknejad, Malihe (2016) Preparation and preliminary studies of 64Cu-antiMUC-1 for breast cancer targeting. Pharmaceutical and Biomedical Research, 2 (2). pp. 31-46.

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Official URL: http://pbr.mazums.ac.ir/article-1-99-en.html

Abstract

PR81 is a monoclonal antibody that binds with high affinity to MUC1 that over expressed on breast tumors. PR81 is considered a suitable targeting molecule that was radiolabeled using Cu-64 for positron imaging studies. The monoclonal antibody was conjugated with DOTA moiety and after purification was evaluated for radiochemical purity, immunoreactivity, cell toxicity and structure integrity as well as biodistribution study in normal rats. The radiolabeled antibody prepared with acceptable radiochemical purity (> 93.2 ± 0.6 , ITLC; specific activity; 4.6 µCi/µg), protein structure integration, significant cytotoxicity and significant immunoreactivity retention was assessed by radioimmunoassay (RIA). Animal biodistribution of the 64Cu-DOTA-PR81 was consistent with other radiolabeled antibodies. The results showed that 64Cu-DOTA-PR81 may be considered for tumor imaging for ultimate diagnosis and follow-up of MUC1 expression in oncology.

Item Type: Article
Uncontrolled Keywords: Copper-64, PR81, MUC1, tumor, biodistribution
Depositing User: Unnamed user with email eprints@mazums.ac.ir
Date Deposited: 04 Jan 2018 09:26
Last Modified: 04 Jan 2018 09:26
URI: http://eprints.mazums.ac.ir/id/eprint/356

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