Growth inhibition of Secreted DNA of human myeloid cell line (HL-60) on peripheral blood lymphocyte

Abolhasani, M and Chiao, J (2004) Growth inhibition of Secreted DNA of human myeloid cell line (HL-60) on peripheral blood lymphocyte. Journal of Mazandaran University of Medical Sciences, 14 (43). pp. 9-17.

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Official URL: http://jmums.mazums.ac.ir/article-1-375-en.html

Abstract

Background and purpose: So far many protein factors capable of inhibiting the immune system are secreted by different cancer cell lines in the culture supernatant and characterized. �n this report, the release of DN� fragments into the culture supernatant of human promyelocytic cell line, HL-60, with immunosuppressive activity was investigated. Materials and methods : HL-60 culture supernatant was concentrated and applied to ion-exchange D���-Sepharose �L-6B column. The column was eluted with Na�l gradient and the fractions were collected for assay. Fractions with immunosuppressive activity were purified with HPL�. Result: The data show that trypsin and RNase have no effect on antiproliferation, but DNase destroyed the immunosuppressive activity. The DN� with irreversible immuno-suppressive activity was found to be double-stranded with the molecular weight range between 0.6 and 1.5 kilobases. This DN� did not change the cell number and viability and as a result was not cytotoxic. �ell cycle analysis has suggested that DN� molecule could arrest the mitogen-stimulated lymphocytes in G1 phase, and prevent the cells from entering into the proliferating S phase. �onclusion: These data show that DN� like immunosuppressive proteins could inhibit the proliferation of lymphocytes as well as the proliferation of some cancer cells.

Item Type: Article
Uncontrolled Keywords: DN�, HL-60 cells,Lyphocytes,Leukemia-myelocytic-�cte-prevention control, immunosu ppressive �gents
Depositing User: Unnamed user with email eprints@mazums.ac.ir
Date Deposited: 04 Jan 2018 18:04
Last Modified: 04 Jan 2018 18:04
URI: http://eprints.mazums.ac.ir/id/eprint/2889

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