Production of Conjugated Linoleic Acid by Transformed E.coli

Farmani, J and Safari, M and Roohvand, F and Aghasadeghi, M.R and Razavi, S.H and Motevalli, F (2011) Production of Conjugated Linoleic Acid by Transformed E.coli. Journal of Mazandaran University of Medical Sciences, 21 (82). pp. 37-46.

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Background and purpose: Due to the beneficial physiological effects of conjugated linoleic acid (�L�), there has been a growing tendency to produce it as a functional lipid in recent years. Different �L� isomers have different physiological effects hence, production of certain �L� isomers with high purity is of great importance. �L� can be produced through both chemical and enzymatic methods however, unlike chemical catalysts, enzymes make it possible to produce pure �L� isomers. �n this study, linoleic acid isomerase from Propionibacterium acnes was expressed in �. coli and the possibility of the production of �L� was studied. Materials and methods: The vector containing linoleic acid isomerase, pG�X-6P-P��, was transformed in �. coli . Transformants were selected based on their resistance to ampicillin and restriction digestion analysis. To express the recombinant linoleic acid isomerase, transformants were induced using isopropyl-beta-thiogalactopyranoside (�PTG). The expression of recombinant protein was confirmed by SDS-polyacrylamide gel electrophoresis and Western blotting using anti-linoleic acid isomerase antibody. Then, the possibility of the production of �L� from Linoleic acid by using �-coli transformant was investigated. Results: Recombinant linoleic acid isomerase was intracellularly produced as a glutathione S-transferase (GST) tagged protein by transformed �-coli. The fusion of GST to the N-terminus of linoleic acid isomerase increased its molecular weight from 49 to 75 kDa. GST-tagged enzyme acted like linoleic acid isomerase and the transformed bacterium could convert considerable amounts of linoleic acid to �L�. �onclusion: The findings indicated that transformed �. coli can be used for �L� production in biocatalytic processes.

Item Type: Article
Uncontrolled Keywords: �onjugated linoleic acid (�L�), linoleic acid isomerase, �. coli, Propionibacterium acnes
Depositing User: Unnamed user with email
Date Deposited: 04 Jan 2018 16:56
Last Modified: 04 Jan 2018 16:56

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