Mahdiyoun, Seyyed Mohsen and Ahanjan, Mohammad and Goudarzi, Mehdi and Rezaee, Razieh (2015) Prevalence of Antibiotic Resistance in Methicillin-resistant Staphylococcus aureus and Determining Aminoglycoside Resistance Gene by PCR in Sari and Tehran Hospitals. Journal of Mazandaran University of Medical Sciences, 25 (128). pp. 97-107.
Full text not available from this repository.Abstract
Abstract Background and purpose: Staphylococcus aureus is one of the major causes of community and hospital-acquired infections. Aminoglycosides are potent bactericidal agents that are often used in combination with beta-lactam or glycopeptide in treatment of staphylococcal infections. The aim of this study was to investigate the prevalence of the aminoglycoside modifying enzyme aac(6Î�)-Ie/aph(2”) genes and mecA. Materials and methods: In current study, 174 clinical isolates of MRSA from different clinical specimens were tested for antibiotic susceptibility using the Kirby Bauer method. MRSA isolates were detected by disc diffusion method using 1 ug oxacillin and 30 ug cefoxitin discs. Then, MRSA strains were further analyzed for detection of mecA and aminoglycoside resistant aac (6Î�)-Ie/aph (2Ë�) genes by PCR. Results: All strains were resistant to oxacillin and cefoxitin. The highest and lowest resistances were found against erythromycin (85.1) and trimethoprim/sulfamethoxazole (24.7), respectively. In PCR, mecA gene was detected in 100 of the strains and 77 of the strains were found to harbor aac(6′)/aph(2//)-Ia gene. Conclusion: According to this study, the prevalence of aminoglycosides resistant genes is increasing in MRSA isolates.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | mecA gene, aac(6�)-Ie/aph(2�) gene, aminoglycoside modifying enzyme, Staphylococcus |
| Depositing User: | Unnamed user with email eprints@mazums.ac.ir |
| Date Deposited: | 04 Jan 2018 18:25 |
| Last Modified: | 04 Jan 2018 18:25 |
| URI: | http://eprints.mazums.ac.ir/id/eprint/2169 |
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