Interaction between C677T and A1298C MTHFR Gene Polymorphisms and Weight Loss Diet in Treatment of NAFLD

Ghaemi, Alireza and Fakheri, Hafez and Rafiei, Alireza and hosseini, Vahid and Homayounfar, Reza and Amiri, Zohreh and AzamTaleban, Fourugh and Hekmatdoost, Azit (2016) Interaction between C677T and A1298C MTHFR Gene Polymorphisms and Weight Loss Diet in Treatment of NAFLD. Journal of Mazandaran University of Medical Sciences, 25 (133). pp. 10-24.

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Background and purpose: Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver dysfunction worldwide. Genetic factors are believed to affect the development of NAFLD. This study was conducted to evaluate the interaction of weight loss and MTHFR polymorphism on NAFLD characteristics. Materials and methods: A single-arm trial was performed in 50 patients. They received a weight loss diet for 6 months. Anthropometric parameters, liver enzymes, dietary intakes, and physical activity were measured at first, and the end of months 3 and 6. Lipid profile, homocysteine, malondialdehyde, TNF-α, IL-6, and cytokeratin 18 were measured at baseline and at the end of the study. C677T and A1298C MTHFR gene polymorphisms were assessed in all patients using PCR-RFLP and Tetra-ARMS methods, respectively. Results: Forty four patients completed the study protocol. According to MTHFR polymorphism the patients were classified as (CC) and (AC) group (n= 35), and (CT+TT) and (AC) group (n= 9). After 3 and 6 months, weight loss occurred equally in both groups. In CC genotype, fasting blood glucose (P= 0.012), total cholesterol (P= 0.001), and LDL (P=0.004) decreased significantly after 6 months. In those with CT+TT genotype, after 6 months, 6.6 weight loss occurred but it could not significantly reduce liver enzymes and the patients’ homocysteine levels were higher than normal range. Conclusion: In this study, no significant reduction in liver enzymes was observed in the CT+TT sub group, therefore, MTHFR gene polymorphisms should be considered in NAFLD management.

Item Type: Article
Uncontrolled Keywords: Non-alcoholic fatty liver disease, diet, weight loss, MTHFR polymorphism
Depositing User: Unnamed user with email
Date Deposited: 04 Jan 2018 18:38
Last Modified: 04 Jan 2018 18:38

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