Influence of Adipose Derived Mesenchymal Stem Cells on the Effective Inflammatory Factors of Diabetic Wound Healing in Animal Models

Ghaneialvar, Hori and Arjmand, Sareh and SahebghadamLotfi, Abbas and Soleimani, Masoud and MashhadiAbbas, Fatemeh (2017) Influence of Adipose Derived Mesenchymal Stem Cells on the Effective Inflammatory Factors of Diabetic Wound Healing in Animal Models. Journal of Mazandaran University of Medical Sciences, 27 (148). pp. 12-21.

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Official URL: http://jmums.mazums.ac.ir/article-1-9950-en.html

Abstract

Background and purpose: Chronic non-healing diabetic wounds are a major medical challenge worldwide. Stem cell therapy has been developed as a new approach in the management of such wounds that need to be explored further. In the present study we aimed to evaluate the effects of adipose derived mesenchymal stem cells (ADSCs) on gene expression level of some important inflammatory factors involved in wound healing in diabetic animal model. Materials and methods: ADSCs were isolated from adipose tissue of mice and their stemness was identified in terms of specific cell surface markers (CD44, CD90, and CD34) and multi-lineage differentiation potentials. Then 106 stem cells were injected in dermis area around the wounds and the wound tissues were tested for the expression level of IL-8 and IL-10 genes and histopathological analysis at 3rd, 7th, 14th, and 21st days after treatment. Results: Expression level of IL-10 gene at days 3, 14 and 21 increased significantly (P <0.05) in the stem cell treated wound tissues compared to that in non-diabetic and diabetic groups without treatment. The expression of IL-8 gene showed reduction at days 7, 14, and 21 in the same treated tissues. Histopathological examination indicated that administration of stem cells led to skin appendages, lack of inflammation in the wound tissue, granulation tissue appearance, and a stronger overall healing. Conclusion: The present study indicated that stem cells could improve the indicators of wound healing.

Item Type: Article
Uncontrolled Keywords: diabetic wound, healing, cytokines, stem cell
Depositing User: Unnamed user with email eprints@mazums.ac.ir
Date Deposited: 04 Jan 2018 18:55
Last Modified: 04 Jan 2018 18:55
URI: http://eprints.mazums.ac.ir/id/eprint/1846

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