Molecular Detection of Zonula Occludens (zot) Toxin Genes in Vibrio Cholerae O1 Using PCR

Mousavi, Seyedeh Mahnaz and Zeinoddini, Mehdi and Azizi, Azadeh and Saeedinia, AliReza and Monazah, Ari (2017) Molecular Detection of Zonula Occludens (zot) Toxin Genes in Vibrio Cholerae O1 Using PCR. Research in Molecular Medicine, 5 (3). 0-0.

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Background: Zot (zonula occludens toxin) is one of the secretion toxins of Vibrio cholerae in small intestine that bind to certain receptors in the epithelial cells and causes a change in the structure of tight junction. The purpose of this research is rapid detection of zot enterotoxin gene using PCR. Materials and Method: The genomic DNA was extracted by DNA isolation kit and gene amplification carried out by the Zot gene-specific primers. Then, PCR products were investigated by electrophoresis on 1.2 agarose gel stained by ethidium bromide. Also, the specificity of primers was measured using bacterial samples other than V. cholerae, such as Escherichia coli enterotoxigenic )ETEC(, Salmonela. typhi, Aeromonas hydrophila and the sensitivity of the PCR reaction was also evaluated using serial dilutions of V. cholerae O1 concentration (cfu/ml). Results: The data was shown that the designed primers specificity for zot gene was successfully, and the sensitivity of this method was determined about 142 cfu/ml. Conclusion: In conclusion, this molecular detection can be used like a simple diagnostic kit in clinical laboratories for identification of V. cholerae.

Item Type: Article
Uncontrolled Keywords: Vibrio cholerae, zot, PCR, Diagnostic.
Depositing User: Unnamed user with email
Date Deposited: 04 Jan 2018 15:09
Last Modified: 04 Jan 2018 15:09

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