Simvastatin and Recombinant Antagonist of Receptors of Interleukin-1 Modulate Toll-like Receptors in Experimental Acute Ileitis in Rat

Zherebiatiev, Aleksandr and Kamyshnyi, Aleksandr (2014) Simvastatin and Recombinant Antagonist of Receptors of Interleukin-1 Modulate Toll-like Receptors in Experimental Acute Ileitis in Rat. Research in Molecular Medicine, 2 (3). pp. 11-16.

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Official URL: http://rmm.mazums.ac.ir/article-1-90-en.html

Abstract

Background: The pathogenesis of inflammatory bowel disease is complex and multifactorial. Studies have led to the current concept that Toll-like receptors represent key mediators of innate host defense in the intestine, and they are involved in maintaining mucosal as well as commensal homeostasis. We studied the possibility of simvastatin and antagonist of receptors of interleukin-1 for pharmacological correction of acute ileitis in rats with a focus on the expression intensity studies of TLR2 TLR4 with lymphocytes of small intestine. Materials and Methods: Experiments were carried out on male Wistar rats aged 5–7 months (body mass 260–285 g). Rats were divided into four experimental groups: group 1 � control group 2 � rats with indomethacin-induced ileitis group 3 � the rats were given simvastatin (20 mg/kg, for 5 days, subcutaneously) group 4 �the rats had given antagonist of receptors of interleukin-1 (3 mg/kg, for 5 days, subcutaneously). The TLR2 and TLR4 immunopositive lymphocytes were determined using a direct immunofluorescence technique using a monoclonal rat antibody. Results: We established that development of ileitis was accompanied with the change of amount of TLR2+ and TLR4+ lymphocytes and the density of TLR2, TLR4 in immunopositive cells. Drug administration during the development of experimental pathology was accompanied by changes in the expression of TLR2, TLR4 and their density on lymphocytes. Conclusions: Simvastatin and antagonist of receptors of interleukin-1 seemed to be beneficial in indomethacin-induced rat ileitis model through modulate TLR2 and TLR4 expression with lymphocytes of small intestine.

Item Type: Article
Uncontrolled Keywords: Ileitis, Recombinant antagonist ,receptors of interleukin-1 (ARIL-1), Simvastatin, Toll-like receptor
Depositing User: Unnamed user with email eprints@mazums.ac.ir
Date Deposited: 04 Jan 2018 14:47
Last Modified: 04 Jan 2018 14:47
URI: http://eprints.mazums.ac.ir/id/eprint/1603

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