Effects of Combination of G-CSF and SCF One Week Prior to Liver Injury In Acute liver Damage Model Induced by Thioacetamide Administration

Yoonesi, Ali Asghar and Qujeq, Durdi and Esmaili, Mohsen and Feizi, Farideh (2014) Effects of Combination of G-CSF and SCF One Week Prior to Liver Injury In Acute liver Damage Model Induced by Thioacetamide Administration. Research in Molecular Medicine, 2 (1). pp. 21-25.

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Official URL: http://rmm.mazums.ac.ir/article-1-66-en.html

Abstract

Background: There are many reports regarding to effects of Granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF)alone in liver repair .But conflicting data have been reported regarding the role of growth factors such as G-CSF and SCF in the liver regeneration system. Also, there is not such data regarding to effects of co-administration both of G-CSF and SCF in the liver damage condition. Materials and Methods: An experimental model of rat liver damage induced by the thioacetamide. Five different groups of animals receiving 0.9 NaCl, TAA alone, TAA + G-CSF, TAA + SCF and TAA + (G-CSF+SCF ). The activity of glutamate pyruvate transaminase (GPT/AlT)and glutamate oxaloacetate transaminase (GOT/AST) were measured after the thioacetamide (TAA) injection and the administration of combination of G-CSF + SCF for 12 weeks. Also histological tests were carried out at the end experiments. Results: The pre-treatment of combination of G-CSF and SCF for 12 weeks reduced the degree of liver injury. The mean of GOT activity was 61.24 (U/L) in the G-CSF +SCF and versus 132.86 in the TAA-alone group. These differences in the GOT activity were statistically significant (P<0.05). Also, in the G-CSF +SCF and TAA group the mean of GPT activity (4.35 versus 11.79, respectively) were lower than in the TAA-alone group, this difference was statistically significant (P<0.05). Liver sections from a rat treated only with TAA, showing damage, but TAA and G-CSF + SCF no significant damage is present. On the other hand histological results revealed a very mild degree of inflammation were observed in the livers of the combination of G-SCF+SCF and TAA-treated rats compared to TAA only treated group. Conclusion: Biochemical and microscopic analysis revealed that combination of G-CSF and SCF pre-treatment significantly enhances liver regeneration after TAA –induced liver injury.

Item Type: Article
Uncontrolled Keywords: Damage, Granulocyte colony-stimulating factor, stem cell factor, thioacetamide, glutamate pyruvate transaminase, glutamate oxaloacetate transaminase
Depositing User: Unnamed user with email eprints@mazums.ac.ir
Date Deposited: 04 Jan 2018 14:29
Last Modified: 04 Jan 2018 14:29
URI: http://eprints.mazums.ac.ir/id/eprint/1584

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