Proteolytic activity and cooperative hemolytic effect of dermatophytes with different species of bacteria

Pakshir, Keyvan and Mohammadi, Tooba and khodadadi, Hossein and Motamedifar, Mohammad and Zomorodian, kamiar and Alipour, Saeideh and Motamedi, Marj (2016) Proteolytic activity and cooperative hemolytic effect of dermatophytes with different species of bacteria. Current Medical Mycology, 2 (4). pp. 9-14.

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Official URL: http://cmm.mazums.ac.ir/article-1-125-en.html

Abstract

Background and Purpose: Globally, dermatophytes are the most common filamentous group of fungi causing cutaneous mycoses. Dermatophytes were shown to secrete a multitude of enzymes that play a role in their pathogenesis. There is limited data on co-hemolytic (CAMP-like) effect of different bacterial species on dermatophyte species. In this study, we sought to the evaluate exoenzyme activity and co-hemolytic effect of four bacteria on clinical dermatophytes isolated from patients in Shiraz, Iran. Materials and Methods: A total of 84 clinical dermatophyte species were isolated from patients suffering dermatophytosis and identified by conventional methods. Hemolytic activity was evaluated with Columbia 5 sheep blood agar. Proteolytic activity was determined by plate clearance assay method, using gelatin 8 agar. CAMP-like factor was evaluated with four bacteria, namely, S. areus, S. saprophyticus, S. pyogenes, and S. agalactiae. Fisher's exact test was run for statistical analysis. Results: T. mentagrophytes was the most predominant agent (27 32.1%) followed by T. verrucosum (20 23.8%), T. tonsurans (10 11.9%), Microsporum canis (7 8.3%), T. rubrum (6 7.1%), E. floccosum (6 7.1%), M. gypseum (5 6%), and T. violaceum (33.6%). The most common clinical area of dermatophytosis was the skin. All the isolates expressed the zone of incomplete alpha hemolysis. All the isolates had CAMP - positive reaction with S. aureus and the other bacteria were CAMP-negative. All the isolates expressed proteolytic activity and no significant differences were noted among diverse genera of dermatophytes and severities of proteolytic activity. Conclusion: This study indicated that hemolysin and proteolytic enzymes potentially play a role in dermatophyte pathogenesis and S. aureus could be considered as a main bacterium for creation of co-hemolytic effect in association with dermatophyte species.

Item Type: Article
Uncontrolled Keywords: CAMP-like, Dermatophyte, Hemolysin, Proteolytic, Trichophyton mentagrophytes
Depositing User: Unnamed user with email eprints@mazums.ac.ir
Date Deposited: 04 Jan 2018 14:05
Last Modified: 04 Jan 2018 14:05
URI: http://eprints.mazums.ac.ir/id/eprint/1538

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