Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis

Abastabar, Mahdi and Hosseinpoor, Susan and Hedayati, Mohammad Taghi and Shokohi, Tahereh and Valadan, Reza and Mirhendi, Hossein and Mohammadi, Rasoul and Aghili, Seyed Reza and Rahimi, Nooshin and Aslani, Narges and Haghani, Iman and Gholami, Sar (2016) Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis. Current Medical Mycology, 2 (4). pp. 1-8.

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Official URL: http://cmm.mazums.ac.ir/article-1-135-en.html

Abstract

Background and Purpose: Hyphal wall protein 1 (HWP1) is an important adhesin which usually is expressed on the germ tube and hyphal surface produced by different Candida species. The hyphal wall protein-coding gene (HWP1) was evaluated as a novel identification and phylogenetic marker in Candida tropicalis, C. orthopsilosis, C. parapsilosis and C. glabrata. Materials and Methods: Initially, four specific primer pairs were designed, and the target was amplified and finally sequenced. A total of 77 Candida isolates from four different species were included in the study. Consensus sequences were used for the evaluation of phylogenetic tree using the CLC Genome Workbench, GENEIOUS, and MEGA softwares and the levels of nucleotide and amino acid polymorphism were assessed. Results: According to the results, the specific amplified fragments of HWP1 gene were useful for the differentiation of four species. Intra-species variation was observed only in C. tropicalis with two DNA types. The phylogenetic tree of Candida species based on the HWP1 gene showed consistency in topology with those inferred from other gene sequences. Conclusion: We found that HWP1 gene was an excellent marker for the identification of non-albicans Candida species as well as the phylogenetic analysis of the most clinically significant Candida species.  

Item Type: Article
Uncontrolled Keywords: Candida species, HWP1 gene, Identification, Phylogenetic analysis
Depositing User: Unnamed user with email eprints@mazums.ac.ir
Date Deposited: 04 Jan 2018 14:06
Last Modified: 04 Jan 2018 14:06
URI: http://eprints.mazums.ac.ir/id/eprint/1537

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