Effects of Quercetin on Signaling Proteins (pSTAT3, pERK1/2, pAKT) and Interleukin-6 Gene Expression in Prostate Cancer PC3 cells

Heidarian, Esfandiar and Amini, Asadollah and Abbasi-Veldani, Akram and Ghatreh-Samani, Keih (2017) Effects of Quercetin on Signaling Proteins (pSTAT3, pERK1/2, pAKT) and Interleukin-6 Gene Expression in Prostate Cancer PC3 cells. Journal of Mazandaran University of Medical Sciences, 26 (144). pp. 290-300.

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Official URL: http://jmums.mazums.ac.ir/article-1-9266-en.html


Background and purpose: Interleukin-6 (IL-6) causes the progression of prostate cancer through pSTAT3, pERK1/2, and pAKT cell signaling proteins. Quercetin, an herbal antioxidant, has anti-tumor effect. The aim of this study was to evaluate the effects of quercetin on IL-6 gene expression, and the above cellular signaling proteins in PC3 prostate cancer cells. Materials and methods: In this experimental study, PC3 cells were treated with different concentrations of quercetin at 0, 10, 50, and 100 μM.  Then, IL-6 concentration was determined in cell culture media. Also, total RNA and the cellular signaling proteins aforementioned were extracted from PC3 and used for determining IL-6 gene expression by quantitative real-time RT-PCR and western blot analysis, respectively. Results: The quercetin IC50 for PC3 prostate cancer cells was 100 μM. Elevation of quercetin concentration in cell culture media increased the IL-6 gene expression and protein synthesis. At 50 and 100 μM of quercetin, IL-6 protein synthesis increased significantly (P<0.05) to 13.36 and 36.86, respectively, compared to those in control. Furthermore, quercetin suppressed pSTAT3, pERK1/2, and pAKT cell signaling proteins at dose concentrations more than 150 μM. Conclusion: The effects of quercetin on PC3 cells could have resulted from reduction of pSTAT3, pERK1/2, pAKT, induction of the oxidative stress and generation of reactive oxygen species. Therefore, quercetin can be considered as a useful therapeutic agent in treatment of prostate cancer.

Item Type: Article
Uncontrolled Keywords: interleukin-6, cell line PC3, prostate cancer, quercetin, pSTAT3, pERK1/2, pAKT
Depositing User: Unnamed user with email eprints@mazums.ac.ir
Date Deposited: 04 Jan 2018 12:18
Last Modified: 04 Jan 2018 12:18
URI: http://eprints.mazums.ac.ir/id/eprint/1496

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